PCR_TaqDNAPolymerase_SOP
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>Utilisation de la TaqDNA Polymérase 5,000 U/ml NEB Biolabs Préparation du Mastermix : pour 1 réaction = Taq Ready Mix 12,5µl Primer F 1,25µl Primer R 1,25µl ADN matrice qsp 0,05µg/µl Eau DEPC qsp 25µl >Programme du thermocycleur : -Heat lid 105°C -Dénaturation initiale 95°C 5minutes 30 cycles de PCR : -Dénaturation 94°C 30secondes -Hybridation 54°C 45secondes -Elongation 72°C 2minutes -Elongation terminale 72°C 5minutes

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Created: 14th May 2018 at 08:07

Last updated: 14th May 2018 at 08:07

Last used: 23rd May 2022 at 23:02

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Version 1 Created 14th May 2018 at 08:07 by Guillaume Audic

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