PCR_TaqDNAPolymerase_SOP
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>Utilisation de la TaqDNA Polymérase 5,000 U/ml NEB Biolabs
Préparation du Mastermix :
pour 1 réaction = Taq Ready Mix 12,5µl
Primer F 1,25µl
Primer R 1,25µl
ADN matrice qsp 0,05µg/µl
Eau DEPC qsp 25µl
>Programme du thermocycleur :
-Heat lid 105°C
-Dénaturation initiale 95°C 5minutes
30 cycles de PCR :
-Dénaturation 94°C 30secondes
-Hybridation 54°C 45secondes
-Elongation 72°C 2minutes
-Elongation terminale 72°C 5minutes

SEEK ID: https://research-sharing.cesgo.org/sops/4?version=1

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Created: 14th May 2018 at 08:07

Last updated: 14th May 2018 at 08:07

Last used: 21st Oct 2021 at 03:25

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