Detection Of QTL and functional genomics study of osmoregulation capacities in the rainbow trout (Oncorhynchus mykiss)

Euryhalin teleosts can live in freshwater as well as in seawater. The success of their survival depends then on their osmotic acclimation capacities. The objective of my work is to describe acclimation processes in the salted water at the rainbow trout by a study coupling functional genomic and genetic approaches. From a first differential gill transcriptomic study, a list of candidates genes was established. This study also allowed to investigate the physiological answer to a hyperosmotic kinetics challenge. Main results reveal good euryhalinity capacities of the tested trouts and a maximum trasncriptomic answer 24 h after the seawater transfer. Biological processes involved in the acclimation mechanisms are also proposed. A second part of this work consisted of the characterisation of the genetic control of processes linked to seawater acclimation in rainbow trout. Using as characters, a two times repeated 24h post seawater transfer plasma sodium and chloride levels, as well as gill weight, unitrait and multitrait analyses allowed to reveal 18 QTL among whom 9 are qualified as robust. A last approach of eQTL detection then allowed, based on a gill transcriptomic analysis and on the results of the first two approaches, to offer 69 exclusive candidates genes. If the majority of these genes are offered as acting at functional level, some are suggested as positional candidates. It is the first time that a blending transcriptomie differential approach coupled to a QTL / eQTL study is led to a non-sequenced genome aquacole interest teleost for the acclimation capacity to different osmotic environments. The existence of a genetic element having an influence on the capacities of osmoregulation in teleost is shown here. These results lay the road with cobblestones for a definite investigation of the genetic bases of seawater acclimation processes in teleosts.

Zenodo URL: https://sandbox.zenodo.org/record/246062

Created at: 5th Oct 2018 at 12:55

Contents

Detection Of QTL and eQTL with effects on osmoregulation capacities in the rainbow trout (Oncorhynchus myhiss)

There is increasing evidence that the ability to adapt to seawater in teleost fish is modulated by genetic factors. Most studies have involved the comparison of species or strains and little is known about the genetic architecture of the trait. To address this question, we searched for QTL affecting osmoregulation capacities after transfer to saline water in a nonmigratory captive-bred population of rainbow trout. A QTL design (5 full-sib families, about 200 F2 progeny each) was produced from a
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Transcription profiling

No description specified

E-coli extraction protocol

SOP for labelled E-coli extraction protocol

  • SOP for labelled E coli extraction.docx

clinical chemistry analysis

No description specified

Data day 2

Chemical Measuments D2

  • Day2_Example_4 Chemical Measurements.xls

data transformed

No description specified

  • guggo.png

Time-course study of rainbow trout Oncorhynchus mykiss functional responses during transfer from freshwater to seawater

90 trouts are transferred from freshwater to seawater during 21 days. Plamsatic ion concentrations and gill transcriptome expression are studied and related to better understand seawater acclimation functional processes on Rainbow trout.

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Created: 5th Oct 2018 at 12:55

Last updated: 5th Oct 2018 at 12:58

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